The Validity of Cross Priming Amplification to Detect SARS-CoV-2 Virus

The standard molecular technique to detect the SARS-CoV-2 virus is Real-Time Reverse-Transcription Polymerase Chain Reaction (rRT-PCR). It requires sophisticated equipment and a time-consuming sample process. Cross Priming Amplification (CPA) nucleic acid amplification that amplifies DNA with high specificity efficiency under constant thermal conditions. This faster than rRT-PCR doesn't require biosafety level-2 (BSL-2) facility. study aimed determine validity of CPA as gold evaluate its performance rapid tests for detecting RNA from nasopharyngeal swab specimens. was descriptive diagnostic test by using data retrospectively patient samples who were treated at Palabuhan Ratu Hospital COVID-19 01 January 31 December 2021. performed on total 52 Pelabuhan Laboratory Provincial Health Laboratory. correlation performed. majority subjects female between ages 34-50 years. cut-off Tt-value 3.25, 0.84 Area Under Curve (AUC), p-value <0.001. has good diagnosis 77% sensitivity, 94% specificity, 96% PPV, 71% NPV. sensitivity increasing Ct-value <30 (82%) <25 (87%). had test. could be used viral